Senior Scientist I, Cell Line Development
Core
Design and test vector constructs, engineer host cells, and conduct transfection/clone-generation campaigns in CHO cells to optimize recombinant antibody expression and titer.
Role type
Senior Scientist I, Cell Line Development (hands-on IC)
Builds
Next-generation CHO cell line platforms for recombinant antibodies and complex biologics
Domain
Biologics / CMC / Molecular Biology
Deliverable
production ML models | product features | dashboards & analysis | research | client delivery | infrastructure | physical/clinical work -> physical/clinical work (specifically: stable CHO cell lines for GMP manufacturing)
Required skills
Vector design and testing, CHO cell engineering (knock-in/knock-out), Transfection, Aseptic mammalian cell culture, Octet (BLI), ViCell, CloneSelect Imager, Microfluidic clone-screening (Beacon), Flow cytometry, ddPCR/RT-qPCR, Geneious Prime, CRISPR/Cas, Recombinant protein expression theory (genomic/epigenetic regulation, transcription, translation, mRNA stability)
Preferred skills
Industry CMC experience, NGS (library prep and analysis), Python/R programming
Technologies
Geneious Prime, Octet, ViCell, CloneSelect Imager, Beacon, CRISPR/Cas
Responsibilities
Design and test dozens of vector constructs for increased mAb titer; Execute transfection, screening, and clone-characterization studies; Operate and interpret data from core CLD instrumentation; Author technical reports and present data to leadership; Collaborate with cell culture, analytical, and bioinformatics colleagues
Seniority
Senior, hands-on IC
