Scientist in Protein Technologies Center - Cell Engineering / Molecular Biology
Core
Engineer cell lines using CRISPR and other genome-editing technologies to generate knockouts, knock-ins, and stable overexpressed lines for structural biology and drug discovery.
Role type
Senior IC molecular biologist (cell engineering)
Builds
Stable cell lines, NGS libraries, and genomic edits for structural cell biology projects
Domain
Biotechnology / Structural Biology / Molecular Biology
Required skills
CRISPR gene editing, molecular cloning (In-Fusion, LIC, PIPE, Gateway, Gibson assembly), site-directed and saturation mutagenesis, non-viral and viral delivery systems, NGS library preparation, genomic stability verification
Preferred skills
PhD in molecular biology or synthetic biology, 5+ years postdoctoral experience
Technologies
CRISPR, In-Fusion, LIC, PIPE, Gateway, FX, overlap extension PCR, Gibson assembly, BioBrick, recombineering, NGS
Responsibilities
Engineer cell lines using CRISPR for gene knockouts and knock-ins; Generate stable cell lines using non-viral and viral-based delivery systems; Implement advanced technologies to develop stable target-overexpressed cell lines and NGS library preparation; Confirm edits and verify genomic stability throughout clonal line-generation.
Seniority
Senior, hands-on IC