Scientist I, Guide RNA Chemistry, Prime Medicine
Core
Synthesize chemically modified guide RNAs using automated synthesizers and phosphoramidite chemistry to support gene editing therapeutic discovery.
Role type
Scientist I, Guide RNA Chemistry
Builds
High-quality guide RNAs for in vivo studies and preclinical development
Domain
Biotechnology / Gene Editing / Oligonucleotide Chemistry
Deliverable
production ML models | product features | dashboards & analysis | research | client delivery | infrastructure | physical/clinical work
Required skills
phosphoramidite chemistry, solid-phase oligonucleotide synthesis, RNA cleavage and deprotection, HPLC purification, UPLC analysis, LC-MS (Orbitrap), UV spectroscopy, enzymatic ligation, linker chemistry, long RNA assembly
Preferred skills
MerMade 12 synthesizer operation, Dr. Oligo synthesizer operation, high-throughput laboratory environment experience
Technologies
MerMade 12, Dr. Oligo, Orbitrap, UPLC, HPLC
Responsibilities
Design and execute complex laboratory experiments for guide RNA synthesis; perform RNA cleavage, deprotection, desalting, and purification; analyze oligonucleotides using UPLC, LC-MS, and UV spectroscopy; troubleshoot synthesis and purification challenges; drive continuous improvements in synthesis workflows; mentor co-op students or junior scientists
Seniority
Entry-level Scientist (0-2 years post-PhD or 6+ years post-MS/BS)