Research Technician II, Gene Editing Core
Core
Support genome editing, CRISPR-based perturbation screening, iPSC reprogramming, and associated molecular and cellular workflows for investigators across MGB and external collaborators.
Role type
Research Technician II (Gene Editing Core)
Builds
Genome-editing and cell therapy research projects
Domain
Biotechnology / Gene & Cell Therapy
Deliverable
production ML models | product features | dashboards & analysis | research
Required skills
mammalian cell culture, DNA/RNA isolation, PCR, gel electrophoresis, basic molecular cloning
Preferred skills
CRISPR-based genome editing, iPSC culture, lentiviral vector production, next-generation sequencing workflows, fluorescence microscopy, flow cytometry, basic sequence analysis
Technologies
CRISPR/Cas9, base editing, prime editing, NGS, GraphPad Prism, Python, R
Responsibilities
Maintain and culture mammalian cell lines including human iPSCs; Perform routine molecular biology procedures including DNA/RNA extraction and plasmid preparation; Support CRISPR-based genome-editing workflows including transfection and genotyping; Support next-generation sequencing workflows including library preparation; Perform cell characterization and quality-control assays; Maintain accurate experimental records and sample inventories; Assist with troubleshooting and optimization of gene-editing workflows.
Seniority
Mid-level, hands-on IC
