Staff / Senior Scientist, Assay Development (Chemistry)
Core
Developing chemical methods for sample loading, conjugation, cleavage, and stabilization in a single-molecule proteome sequencing platform.
Role type
Staff / Senior Scientist, Assay Development (Chemistry)
Builds
Chemical workflows and reagents for the ProSE sequencing assay
Domain
Biotechnology / Proteomics / Chemistry
Deliverable
production ML models | product features | dashboards & analysis | research | client delivery | infrastructure | physical/clinical work
Required skills
peptide chemistry, bioorthogonal and click conjugation strategies, cleavage chemistry development, site-selective bioconjugation, multi-step workflow design, analytical characterization (HPLC, LC-MS/MS, UV-Vis), method development
Preferred skills
nanopore or single-molecule sequencing chemistry, solid-phase peptide synthesis (SPPS), statistical and DOE-based experimental design
Technologies
HPLC (RP and IEX), LC-MS/MS, UV-Vis, CE
Responsibilities
Optimize bioconjugation methods for peptide loading into the expansion assay; Identify, characterize, and optimize peptide labeling during the ProSE sequencing assay; Design and determine the order of operations for chemical manipulations within the assay workflow; Optimize selective cleavage chemistry workflows for use within the sequencing assay; Employ analytical methods to characterize conjugation efficiency, adduct identity, purity, and stability of assay intermediates and conjugates
Seniority
Senior/Staff Scientist, hands-on IC