Research Assistant III Lab
Core
Construct high-quality nascent RNA libraries for Next Generation Sequencing (NGS), optimize library construction methods, and analyze sequencing data to reveal direct targets of gene regulation.
Role type
Research Assistant III (Molecular Biology/Lab Services)
Builds
High-quality NGS libraries for nascent RNA analysis
Domain
Biomedical research / Molecular biology / Genomics
Required skills
Molecular cloning techniques, PCR/qPCR, nucleic acid ligation, buffer preparation, RNase-free techniques, NGS library construction (RNA-seq, ChIP-seq), mammalian tissue culture, aseptic technique, Linux command line/shell scripting, R, bioinformatics for NGS data analysis
Preferred skills
Method optimization design, protocol improvement, sample preparation advice
Technologies
NGS, qPCR, TapeStation, gel electrophoresis, Linux, R
Responsibilities
Receive and perform quality control (QC) on cells and materials; Construct NGS libraries according to established protocols; Perform QC on constructed libraries using qPCR, TapeStation, gel electrophoresis; Analyze users' data following established pipelines and scripts; Interpret analysis results and investigate potential problems; Prepare data packages for users; Maintain detailed, accurate, up-to-date records of all samples and experiments; Devise and execute experiments to test protocol improvements in consultation with the core director.
Seniority
Mid-level IC (2+ years experience) (via careerplan.io/jobs/3743990013872276-research-assistant-iii-lab-at-harvard-university)