Scientist 3 - Nucleic Acids Payload Engineering
Core
Lead efforts to develop non-viral gene delivery platforms by engineering nucleic acid payloads for nuclear delivery and mitigating immunogenicity.
Role type
Senior IC molecular biologist (gene delivery engineering)
Builds
Novel modalities for nuclear delivery of donor DNA cargo using Super PiggyBac transposase-based systems
Domain
Biopharma / Gene Therapy / Nucleic Acid Biology
Required skills
high-throughput library screening in mammalian cell systems, nucleic acid manipulation, molecular cloning (Golden Gate, Gibson assembly), IVT, mammalian expression vector design, functional molecular assays (Western blot, ddPCR, qRT-PCR)
Preferred skills
Next-Generation Sequencing (NGS), work with primary cells (hepatocytes, T cells, monocytes/macrophages)
Technologies
Super PiggyBac transposase, Golden Gate cloning, Gibson assembly, ddPCR, qRT-PCR
Responsibilities
Conceptualize and evaluate novel strategies for nuclear shuttling of DNA, perform exploratory research using hypothesis testing and screening methodologies, collaborate with cross-functional teams to convert research data into clinical and drug discovery frameworks
Seniority
Senior, hands-on IC