Scientist, Eukaryotic Genome Engineering
Core
Engineer eukaryotic genomes end-to-end, focusing on mammalian tissue culture, from design and reagent production to verified edit confirmation.
Role type
Scientist, Eukaryotic Genome Engineering
Builds
Verified edited mammalian cell lines and clones
Domain
Molecular biology / Genome engineering / Mammalian cell culture
Deliverable
production ML models | product features | dashboards & analysis | research | client delivery | infrastructure | physical/clinical work
Required skills
CRISPR and variants (base, prime, ZFNs, TALENs), mammalian tissue culture, DNA/RNP delivery (transfection, electroporation, nucleofection), NGS-based verification, cloning, PCR, plasmid prep, single-cell sorting (FACS), genotype-to-phenotype validation
Preferred skills
Viral vector production (lentivirus, AAV), iPSC and primary-cell culture, high-throughput CRISPR screening, off-target profiling, bioinformatics scripting
Technologies
Benchling, SnapGene, CRISPResso2, ICE, TIDE, Illumina, ddPCR, Western blot, qPCR, flow cytometry, immunofluorescence
Responsibilities
Design editing strategies and reagents; Build and QC editing reagents; Maintain mammalian tissue culture; Deliver editing machinery via transfection/electroporation/viral vectors; Optimize nuclease toolkit efficiency; Isolate and expand edited clones; Verify edits by NGS; Validate genotype-to-phenotype
Seniority
Senior, hands-on IC
