Cell Assay Lead/Principal Scientist - Blue Sky Kinases
Core
Design and perform cell-based assays to translate in vitro kinase structural insights into functional cellular effects, validate structural findings, and discover new kinase-mediated biology.
Role type
Senior Scientist (Cell Biology/Assay Development)
Builds
Cell-based assays for kinase signaling, protein phosphorylation, and cellular phenotypes
Domain
Structural Biology / Kinase Signaling / Cell Biology
Required skills
Cell signaling research, Cell culture (various lines), Exogenous protein expression, Statistical analysis, Experimental design, Troubleshooting
Preferred skills
Mouse models
Technologies
qRT-PCR, ELISA, AlphaLISA, Mesoscale Discovery, Immunoprecipitation, Western blotting, FRET, NanoBit, Flow cytometry, FACS, Reporter gene assays, Immunofluorescence, Live-cell imaging, Cellular fractionation
Responsibilities
Design and perform experiments translating in vitro kinase insights to cellular effects; Guide junior staff in experimental design and data review; Develop strategies for a wide spectrum of cell-based assays (phosphorylation, interaction, localization, etc.); Lead collaboration on structure-guided cell biology projects; Implement and troubleshoot phenotypic assays (viability, apoptosis, migration, etc.); Analyze assay data using statistical software or R scripts; Interpret literature and experimental data; Contribute to team growth and mentoring
Seniority
Senior, hands-on IC with mentorship responsibilities