STAGE Laboratoire analyse des eaux (6 mois) - Microbiologie, Biologie Moléculaire ou Biotechnologie F/H
Core
Develop and optimize a new qPCR protocol on the QuantStudio 5 thermocycler for monitoring swimming water, focusing on reducing detection time and improving sensitivity for E. coli and Enterococci.
Role type
Junior IC molecular biologist (water analysis)
Builds
Optimized qPCR protocols and standard operating procedures for water quality monitoring
Domain
Environmental water analysis + molecular biology
Required skills
qPCR protocol development, QuantStudio 5 operation, TaqMan probe design, oligonucleotide primer design, multiplexing, reaction parameter optimization, laboratory metrology
Preferred skills
Permis B (driving license)
Technologies
QuantStudio 5, TaqMan probes, qPCR
Responsibilities
Adapt qPCR method to QuantStudio 5, optimize reaction parameters and calibration range, design specific probes and primers for multiplexing, reduce total reaction time, write new operating procedures, validate protocol with real samples, participate in regular lab activities
Seniority
Intern (6 months)