Data Science Fellow — Organoid Biochemistry
Core
Establish core capacity for protein-focused biological support, connecting organoid phenotype and optical measurements to molecular mechanisms.
Role type
Postdoctoral Fellow (Biochemistry/Protein Engineering)
Builds
Protein/nanobody libraries, membrane-active-polymer workflows, native nanodiscs, and biochemical assays for organoid research.
Domain
Biomedical research, organoid biology, protein chemistry, membrane biology.
Required skills
recombinant protein expression, protein purification, molecular cloning, quantitative binding assays, protein-interaction analysis, Python/R/MATLAB data analysis, SOP documentation
Preferred skills
nanobody engineering, display technologies, FACS, next-generation sequencing, cell-free protein synthesis, microfluidics, hydrogel beads, styrene-maleic-acid polymers, SMALPs, native nanodiscs, lipidomics, mass spectrometry, electron microscopy, organoid models, toxicology, fluorescence imaging
Technologies
Python, R, MATLAB, FACS, NGS, ELISA, SPR, SEC, DLS, negative-stain EM, mass spectrometry
Responsibilities
Establish routine molecular-biology, protein-expression, purification, conjugation, and biochemical-assay support for organoid projects; Implement APB-Display using dual-functional hydrogel beads, pooled DNA libraries, particle-templated emulsification, emulsion PCR, emulsion in-vitro transcription/translation, SNAP-mediated protein capture, fluorescence-based expression and binding measurements, FACS sorting, and sequencing-based readout; Develop focused nanobody and affinity-reagent libraries informed by in-silico design, biological targets, chemical-toxicity hypotheses, and needs identified by the imaging and spectroscopy researchers; Implement APB-SortSeq for rapid single-concentration screening and APB-TiteSeq for quantitative concentration-series measurements of expression and binding affinity; Validate selected binders and interaction measurements with orthogonal methods such as fluorescence polarization, ELISA, pull-down/immunoprecipitation, biolayer interferometry, surface plasmon resonance, size-exclusion chromatography; Establish SMA/SMALP and related membrane-active-polymer screening in relevant cell lines, organoid-derived cells, tissue preparations, and organoid models; Apply quality-control methods for native nanodiscs, including fluorescence measurements, dynamic light scattering, size-exclusion chromatography, negative-stain electron microscopy, gel-based analysis, and mass-spectrometry-compatible preparation; Coordinate targeted extraction and affinity enrichment of membrane proteins and complexes from plasma membrane, mitochondria, endoplasmic reticulum, Golgi, lysosomal, or other organellar membranes; Develop nanobody-enabled labeling, concentration, purification, and perturbation workflows for organoid imaging, membrane-complex analysis, and potential countermeasure applications; Create version-controlled SOPs, reagent inventories, assay acceptance criteria, sample metadata, and training modules; Cross-train other team members in routine biochemical procedures and troubleshoot biologically driven variation in imaging or spectroscopy studies.
Seniority
Postdoctoral Fellow (early-career researcher)