Research Assistant (Environmental Health and Engineering)
Core
Support laboratory research focused on developing alternatives to animal testing, specifically neurotoxicity and developmental neurotoxicity linked to autism and neurodegeneration.
Role type
Research Assistant (Laboratory Operations & Cell Culture)
Builds
In vitro 3D brain organotypical cultures (brain organoids), iPSC culture, electrophysiology, and toxicity testing models.
Domain
Environmental Health and Engineering / Neuroscience / Toxicology
Deliverable
physical/clinical work
Required skills
iPSC culture, 3D brain organoid maintenance, aseptic technique, RNA isolation, Real-Time PCR, cell passaging, cryopreservation, media preparation, immunohistochemistry, laboratory maintenance
Preferred skills
Stem cell and organoid experience, molecular biology techniques, gene editing (CRISPR/Cas9), microphysiological systems knowledge, neuroscience background, electrophysiology
Technologies
iPSC, 3D brain organoids, Real-Time PCR, Western blot, CRISPR/Cas9, organ-on-chip platforms
Responsibilities
Maintain iPSC, neuroprogenitor cells, and 3D brain organoid cultures; perform general laboratory maintenance and cell culture work; conduct immunohistochemistry, RNA isolation, and Real-Time PCR; prepare media, buffers, reagents, and solutions.
Seniority
Entry-level, hands-on IC