Research Lab Technician II
Core
Support interdisciplinary research on G protein-coupled receptor (GPCR) biology and pharmacology using cryo-EM, cell-based assays, and computational modeling.
Role type
Research Lab Technician II (Structural Biology/Pharmacology)
Builds
Production of protein samples and data for GPCR structural studies and drug discovery
Domain
Academic research, Structural Biology, Pharmacology, Cryo-EM
Deliverable
production ML models | product features | dashboards & analysis | research | client delivery | infrastructure | physical/clinical work
Required skills
Recombinant protein expression (insect/mammalian), Protein purification, FRET/BRET assay execution, Molecular biology (cloning/DNA isolation), Cryo-EM sample preparation, Lab reagent preparation
Preferred skills
Single-particle cryo-EM analysis, Cell-based signaling assays, Molecular dynamics simulations, Structure-based drug design
Technologies
Sf9 expression system, Mammalian expression system, Cryo-EM, FRET sensors, BRET sensors, Molecular dynamics software
Responsibilities
Recombinant expression of human proteins in insect and mammalian cell cultures, Perform pharmacological assays using FRET- and BRET-based sensors, Plan and fulfill protein purification, Conduct molecular biology techniques including DNA isolation and cloning, Prepare samples for single particle cryoEM, Prepare lab reagents and chemical solutions, Maintain lab organization and safety compliance
Seniority
Mid-level technical staff, 2+ years experience
