Biochemist II
Core
Create, maintain, and test Master and Working Cell Banks of antibody-producing hybridomas; purify and formulate monoclonal antibodies and conjugates in a regulated, high-volume manufacturing environment.
Role type
Manufacturing Biochemist (Monoclonal Antibody Production)
Builds
Antibody reagents, conjugates, and purified materials for medical diagnostics and research.
Domain
Life Sciences / Biotechnology / Medical Diagnostics
Required skills
Cell culture techniques, Monoclonal antibody purification, Column chromatography, Reagent formulation, GMP compliance, SAP data entry, Equipment maintenance (FPLC/HPLC), Quality reporting (OOS/Deviation), SOP development
Preferred skills
FPLC/HPLC/UV monitor expertise, Kinetic reaction understanding, Regulatory training (FDA/ISO/OSHA)
Technologies
SAP, FPLC, HPLC, UV monitors, Electrophoresis, Size-exclusion columns, CO₂ incubators, Centrifuges, Biosafety hoods
Responsibilities
Create and test Master and Working Cell Banks of antibody-producing hybridomas; Purify monoclonal antibodies using serum fractionation and column chromatography; Conjugate antibodies with fluorophores or dyes; Perform in-process testing for viability, secretion, and sterility; Formulate purified materials and conjugates according to finished-goods specifications; Maintain chromatography systems and equipment; Generate quality reports and participate in investigations; Maintain detailed production records and inventory control.
Seniority
Entry-level to Mid-level Manufacturing Scientist
