AMBER Postdoctoral Fellowship: Next generation vitrification workflows for cryo-ET - Enabling high-throughput cryo-imaging across the microbial tree of life
Core
Developing next-generation sample preparation workflows for high-throughput cryo-electron tomography (cryoET) to visualize cellular ultrastructure in diverse microbial species.
Role type
Postdoctoral Research Fellow (Cryo-ET Method Development)
Builds
Standardized vitrification and multiplexed sample preparation protocols for microbial cell biology.
Domain
Structural biology / Microbial cell biology / Cryo-electron microscopy
Deliverable
production ML models | product features | dashboards & analysis | research | client delivery | infrastructure | physical/clinical work
Required skills
Cryo-electron tomography (cryoET), Vitrification techniques, Focused ion beam (FIB) milling, Microfluidics, Jet vitrification, Correlative imaging, Data analysis
Preferred skills
Serial-lift out cryo-FIB-SEM, Micropatterning of grids, Comparative cellular architecture studies
Technologies
CryoFIB, CryoET, Microfluidic encapsulation
Responsibilities
Integrate microfluidic encapsulation with jet vitrification and cryoFIB/ET imaging; Standardize vitrification across diverse microbial species; Enable multiplexed preparation of different samples on a single EM grid; Develop high-throughput processing workflows for phylogenetically diverse microbes.
Seniority
Postdoctoral Fellow (0-8 years post-PhD)