Protein Purification & Expression Scientist
Core
Support early discovery by providing protein vaccine candidates and assay reagents through small-scale screening and large-scale purification of recombinant proteins and nanoparticles.
Role type
Scientist, protein expression and purification
Builds
Protein vaccine candidates and critical assay reagents
Domain
Biopharmaceuticals / Recombinant protein production
Required skills
Affinity-based FPLC protein purification, Charge-based protein separation, Size-exclusion chromatography, Bradford protein quantitation, UV-Vis spectroscopy, SDS-PAGE, Western blotting, Aseptic technique, Sterile cell culture, Buffer preparation, Instrument maintenance, Record-keeping
Preferred skills
HPLC or UPLC protein characterization, Automated tip-based chromatography, Bacterial or mammalian cell culture, Cell passaging and counting, Orthogonal protein characterization (MS)
Responsibilities
Prepare buffers and maintain instruments, Sanitize chromatographic columns, Track work orders and maintain inventory, Perform small-scale (30 mL) screening of protein antigens, Execute large-scale (5 L) purification of recombinant proteins and nanoparticles, Collaborate with protein characterization and other teams
Seniority
Mid-level Scientist (3-5 years post-grad or 1-2 years grad+)