Research Officer
Core
Lead biochemical reconstitution and characterisation of membrane protein complexes (Wnt signalosome) for structural studies and drug discovery.
Role type
Postdoctoral Research Fellow (Biochemist)
Builds
Research data and manuscripts on membrane protein assembly mechanisms
Domain
Biomedical research / Structural biology / Biochemistry
Deliverable
research
Required skills
Membrane protein expression and purification, Detergent chemistry, Protein complex stabilisation, Molecular biology (cloning/mutagenesis), Cryo-EM or X-ray crystallography sample prep, Biophysical characterisation (SEC-MALS, mass photometry)
Preferred skills
FACS, CRISPR editing, Fluorescence/luminescence pharmacology, Computational structural analysis (PyMOL, AlphaFold)
Responsibilities
Design and optimise expression/solubilisation/purification strategies for membrane complexes; Evaluate detergent strategies for protein integrity; Stabilise large heterogeneous complexes; Interpret PDB data to guide construct design; Prepare samples for cryo-EM or X-ray crystallography; Maintain experimental records and contribute to manuscripts.
Seniority
Postdoctoral Research Fellow