Assistant ingénieur en bioingénierie 3d F/H
Core
Producing 3D human skin models and maintaining primary human cell cultures for the study of cutaneous viruses.
Role type
Research technician (cell culture and tissue engineering)
Builds
3D reconstructed human skin models
Domain
Biomedical research / Tissue engineering
Deliverable
physical/clinical work
Required skills
Primary human cell culture, 3D skin model production, histology preparation, flow cytometry, fluorescence microscopy, PCR, Western Blot, nucleic acid extraction, cell transfection
Preferred skills
Experience with 3D culture, tissue engineering, or microfluidics
Technologies
ImageJ/Fiji, GraphPad Prism
Responsibilities
Amplify, maintain, and cryopreserve primary human cells (keratinocytes, dermal fibroblasts, endothelial cells); prepare culture media and reagents; perform transfections; prepare samples for histological staining and immunostaining; detect viral and cellular proteins via flow cytometry; acquire and quantify fluorescence microscopy images; perform RT-qPCR and Western Blot analyses.
Seniority
Junior to Mid-level, hands-on IC