Gene Editing Lab Technician (2nd Shift)
Core
Perform enzymatic digestion of plant tissues to isolate viable protoplasts, transfect them using PEG-mediated transfection or electroporation, and culture them to regenerate callus.
Role type
hands-on lab technician (plant gene editing)
Builds
plant variety development pipelines
Domain
agriculture + plant biotechnology
Required skills
protoplast isolation, transfection, cell culture, microscopy, data recording, sample preparation
Preferred skills
sterile technique, micropropagation, media preparation, DNA extraction
Technologies
PEG-mediated transfection, electroporation, ImageJ, GraphPad Prism, Excel
Responsibilities
Perform enzymatic digestion of plant tissues to isolate viable protoplasts; Transfect protoplasts using PEG-mediated transfection or electroporation methods; Culture transfected protoplasts to regenerate callus; Assess protoplast yield, viability, and cell health using fluorescence and confocal microscopy; Maintain accurate electronic lab notebooks (ELN) and batch records; Prepare samples for downstream molecular analyses including RNA and DNA extraction.
Seniority
Entry-level to Junior, hands-on IC