Senior Scientist I, Cell/Molecular Biology
Core
Design and test vector constructs, engineer host cells, and conduct transfection and clone-generation campaigns in CHO cells to improve titer in stable cell lines for recombinant antibodies and complex biologics.
Role type
Senior Scientist I, Cell/Molecular Biology (CHO Cell Line Development)
Builds
Next-generation CHO cell line platforms for the production of recombinant antibodies and complex biologics
Domain
Biopharmaceuticals / Cell Biology / Recombinant Protein Expression
Required skills
CHO cell culture, transfection, vector design, gene copy number analysis, RNA expression analysis (ddPCR/RT-qPCR), mAb titer measurement, cell engineering (knock in/out), flow cytometry, Octet (BLI), ViCell, CloneSelect Imager, microfluidic clone screening
Preferred skills
Industrial CMC/Drug-substance environment experience, CRISPR/Cas cell engineering, substantial experience developing CHO expression platform improvements
Technologies
Geneious Prime, Octet, ViCell, CloneSelect Imager, Beacon, ddPCR, RT-qPCR
Responsibilities
Independently conceive and design experiments to investigate CHO expression technical improvements; Execute transfections, screening, and clone-characterization studies; Operate and interpret data from core CLD instrumentation; Author technical reports and present data to the group and leadership; Collaborate with cell culture, analytical, purification, and bioinformatics colleagues
Seniority
Senior, hands-on IC