Wissenschaftlicher Mitarbeiter (m/w/d)
Core
Design and validation of CRISPR/Cas9 libraries, execution of pooled screens, and multi-omics data analysis in human trophoblast stem cell lines.
Role type
Research scientist (CRISPR screening & multi-omics)
Builds
Production CRISPR libraries, clonal cell lines, and integrated multi-omics datasets
Domain
Biomedical research / Molecular biology / Stem cell biology
Required skills
CRISPR/Cas9 technology, mammalian cell culture, stem cell culture (mESC/hiPSC), Next-Generation Sequencing (NGS), R programming, HPC cluster usage, CUT&Tag/ChIP assays, grant writing
Preferred skills
Bioinformatics collaboration, teaching experience, German language skills
Technologies
CRISPR/Cas9, FACS, NGS, R, HPC clusters, CUT&Tag, ChIP
Responsibilities
Design and quality control of pooled gRNA-CRISPR/Cas9 libraries; Establish and culture Cas9-expressing human trophoblast stem cell lines; Conduct pooled screens including large-scale cell culture and FACS-based analyses; Prepare samples for transcriptomic, metabolomic, and multi-omics analyses; Generate and validate clonal cell lines with single or double knockouts; Perform chromatin profiling via CUT&Tag or ChIP assays; Analyze and integrate screen and multi-omics data with bioinformatics partners; Supervise and instruct Master's students; Contribute to scientific publications and presentations; Secure external funding (grants and fellowships); Participate in teaching activities
Seniority
Mid-level researcher (Postdoc level)